FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064298
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26400.248050827869638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52150.040.7754331
GGTATCA20400.040.013631
GTGGTAT7300.039.9353261
TATCAAC58600.036.210062
TGGTATC8400.035.0958182
TCAACGC61000.034.502144
ATCAACG61300.034.3607443
CAACGCA62800.033.6624645
AACGCAG65650.032.305326
ACGCAGA70650.030.0190267
CGCAGAG70700.029.9977978
AGAGTAC73100.029.20009611
CAGAGTA73950.028.86446410
GCAGAGT74300.028.6364159
TCGCTAT854.702044E-628.19312324
ACCGGAC500.007149615328.04832552
GAGTACG42950.025.40648712
AGTACGG43150.025.29110113
GAGTACT41500.025.0577812
AGTACTT42600.024.4130413
Produced by FastQC (version 0.11.5)