FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986391
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46560.47202377150643104No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33390.3385067382001661No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31090.31518941271767487No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28780.2917707075591728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28010.28396447250633877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26550.2691630398087574No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG26340.26703406661252993No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25640.25993748929177174No Hit
CTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25390.2574029973915009No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC24720.2506105590987752No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG24410.24746778914243947No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT24150.24483191756615785No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA23490.23814085894944295No Hit
GTCCCTGACAGGTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT22970.23286911579687972No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22620.22932082713650065No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21950.22252838884377493No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT21570.21867596115536336No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21170.2146207741149301No Hit
GTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTT21050.21340421800280013No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCACT20120.2039759081337928No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCC19590.19860278530521872No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19570.19840002595319706No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18790.1904924112243522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18440.1869441225639731No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAAACT17510.17751581269496577No Hit
GGCCAAAAGTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTG16930.17163579148633756No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA16380.16605990930574183No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15980.16200472226530857No Hit
GTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15920.1613964442092436No Hit
CTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT15890.1610923051812111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.1607881661531786No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15540.157544016520832No Hit
GGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA15160.15369158883242043No Hit
GCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT15140.15348882948039874No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15110.15318469045236624No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14540.14740604891974887No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14390.1458853537795864No Hit
ATCCAGTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGA14360.1455812147515539No Hit
CTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT14350.14547983507554307No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGA14220.14416189928740225No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCCCTGCCCGTCACCC14220.14416189928740225No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13970.14162740738713148No Hit
GAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAA13860.1405122309510123No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13810.14000533257095818No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13580.13767360002270904No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13390.13574738617850327No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13380.13564600650249242No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13220.1340239316863191No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCT13190.13371979265828662No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13190.13371979265828662No Hit
CTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT13120.1330101349262108No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12890.1306784023779617No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12860.1303742633499292No Hit
GTGTAAAATCTGTGCCTGATCCAGTGCCACTGAACCTGTCAGGGACCCCG12770.1294618462658317No Hit
CCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG12770.1294618462658317No Hit
GCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC12540.1271301137175826No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12130.1229735470011385No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12070.1223652689450735No Hit
CCCCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG12010.12175699088900852No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCC11850.1201349160728352No Hit
AGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA11840.12003353639682439No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT11790.11952663801677021No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA11620.11780318352458609No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11620.11780318352458609No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11520.11678938676447778No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11360.11516731194830447No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG11230.11384937616016368No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11160.11313971842808784No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11160.11313971842808784No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGG11100.11253144037202285No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11100.11253144037202285No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10880.11030108749978457No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGGGGAGA10860.1100983281477629No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10760.10908453138765459No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10730.1087803923596221No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10510.10655003948738381No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT10450.10594176143131882No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTC10420.10563762240328632No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10360.10502934434722133No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10190.1033058898550372No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9910.10046725892673392No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9880.10016311989870144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4000.051.5548171
TGGTATC4250.048.5221822
GGTATCA13300.045.4819451
AGTCCGA404.823013E-543.39133545
GTATCAA36000.041.816691
ACGCTAA1006.2855142E-638.53229570
TCGTATG753.9153747E-836.9100940
TATCAAC40900.036.5547642
TCAACGC44150.034.175264
ATCAACG44350.033.943653
CAACGCA45050.033.5688065
AACGCAG45650.033.1275946
ACGCAGA48600.031.1120327
CGCAGAG49600.030.4847728
AACGCTA853.4629324E-530.03362769
AGAGTAC50500.029.80538211
CAGAGTA51950.029.17191910
ACGTAGA2600.029.11024727
GACGTAG2600.029.10729226
AGACGTA2650.028.555225
Produced by FastQC (version 0.11.5)