FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986391
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42450.4303567246659793No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27500.27879410902978635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27190.2756513390734506No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA26930.273015467497169No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25360.25709885836346846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24310.24645399238233118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24150.24483191756615785No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24100.24432501918610366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23390.23712706218933466No Hit
CTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22880.23195669871278224No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22830.2314498003327281No Hit
GTCCCTGACAGGTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT22740.23053738324863063No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT22470.22780013199633817No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG22370.22678633523622985No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC21200.21492491314296255No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20000.20275935202166284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18920.191810347012493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18350.18603170547987563No Hit
GTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTT17930.18177375908742072No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCACT17690.17934064686316076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17280.17518408014671666No Hit
CTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT17010.17244682889442423No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16840.17072337440224009No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCC16790.17021647602218593No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16170.1639309361095144No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15990.1621061019413194No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15650.15865919295695113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15550.15764539619684284No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15400.15612470105668036No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAAACT15210.15419848721247456No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14400.14598673345559723No Hit
GTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG14200.1439591399353806No Hit
CTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT14070.1426412041472398No Hit
GCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT14030.14223568544319648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13980.1417287870631423No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13940.14132326835909897No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13900.14091774965505566No Hit
GGCCAAAAGTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTG13890.14081636997904484No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13820.140106712246969No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13780.13970119354292568No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGA13770.13959981386691486No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13690.1387887764588282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13530.1371667016426549No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCC13430.13615290488254658No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13420.13605152520653574No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCT13170.13351703330626497No Hit
CTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT13170.13351703330626497No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12870.13047564302594003No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCCCTGCCCGTCACCC12820.12996874464588587No Hit
GAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAA12650.12824529015370173No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12480.1265218356615176No Hit
CCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG12240.12408872343725764No Hit
GGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA12230.1239873437612468No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12070.1223652689450735No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11980.12145285186097603No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA11670.11831008190464024No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11640.11800594287660776No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11470.11628248838442364No Hit
GCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC11360.11516731194830447No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTC11180.1133424777801095No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10910.11060522652781707No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10900.11050384685180623No Hit
ATCCAGTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGA10850.10999694847175208No Hit
GTGTAAAATCTGTGCCTGATCCAGTGCCACTGAACCTGTCAGGGACCCCG10630.10776659559951379No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10450.10594176143131882No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT10440.10584038175530798No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA10440.10584038175530798No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10370.10513072402323217No Hit
CCCCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG10340.10482658499519966No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10270.10411692726312385No Hit
AGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA10190.1033058898550372No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA10000.10137967601083142No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9990.10127829633482058No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC9970.1010755369827989No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9900.1003658792507231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4850.046.0262761
GGTATCA15550.041.5204161
TGGTATC5550.040.8192062
GTATCAA39050.040.015161
TATCAAC43300.035.9899752
TCAACGC46050.033.870564
ATCAACG46000.033.8551753
CAACGCA46950.033.1365055
AACGCAG48000.032.554436
GGATCGT450.004597891630.69995738
CGCAGAG51500.030.2754528
ACGCAGA51750.030.261637
AGAGTAC52500.029.69727111
CAGAGTA53600.029.1532210
GTAACAC854.629461E-628.2491993
GATCGTT500.007655233627.65822439
ACGAATT500.00796188627.43650818
AACGCGG658.9087867E-426.40805626
AGTACGG37500.025.86602613
GAGTACG38100.025.54217112
Produced by FastQC (version 0.11.5)