FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015269
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63400.6244650432545463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.31420244289936955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.13070427640359353No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.1305072842763839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12450.12262759918799845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.10883815028332393No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.10214041795819631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9050.043.9996871
GGTATCA34050.042.979731
TGGTATC9850.041.462672
GTATCAA90450.040.6375471
TATCTCG1900.039.7939436
TATCAAC102200.036.0986252
CTTATAC6700.035.5578541
ACCGATC6100.034.6533427
ACGAGAC6150.034.3309422
TATCGCG1850.033.43864436
AGACCGA6400.033.0126725
CCCACGA6500.032.996519
TCAACGC111850.032.9841734
ATCAACG112250.032.8969543
GACCGAT6450.032.7632226
CAACGCA114850.032.122595
AACGCAG116450.031.710466
GAGACCG6800.031.06308724
ATCTCCG7300.030.75980810
CCACGAG7000.030.15772220