FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748156
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.18264960335347646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69950.040.5354231
GTGGTAT8750.040.5064661
GGTATCA28550.039.5332831
TGGTATC9800.036.1664852
TATCAAC78600.035.8555642
ATCAACG83350.034.0186273
TCAACGC83800.033.7538224
CAACGCA86750.032.8445
AACGCAG87200.032.5561186
ACGCAGA94450.030.1560527
CGCAGAG95350.029.7992558
AGAGTAC97900.029.26903511
CAGAGTA101150.028.22658210
GCAGAGT100950.028.1462029
ATCGCGT500.00768128427.64017137
GAGTACT56800.025.1936512
AGTACTT57350.024.95203813
AGTACGG58200.024.7649313
GAGTACG59150.024.3090312
CTAATCG750.001990885523.0050634
Produced by FastQC (version 0.11.5)