FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748156
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43210.24717473726601058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.11097407782829449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76900.041.512451
GTGGTAT10500.039.314131
GGTATCA30950.039.1235921
TATCAAC87250.036.566032
ATCAACG91350.034.8632473
TCAACGC91200.034.8559574
TGGTATC11900.034.090262
CAACGCA93900.033.8728185
AACGCAG95350.033.570746
ACGCAGA103550.030.9785757
CGCAGAG105350.030.514418
AGAGTAC107750.030.15314711
CAGAGTA110750.029.27439910
GCAGAGT110450.029.2607279
AGTACGG67150.026.51832613
GAGTACG68700.025.86860312
GAGTACT57950.025.16143612
AGTACTT58750.024.52822713
TACGGGG37350.023.7043715
GTACGGG76700.022.9059914
Produced by FastQC (version 0.11.5)