FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970847
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA22440.2311383771078244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16710.17211774872868743No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG16680.1718087402031422No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC15630.1609934418090595No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC15200.15656431960957803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.15563729403294238No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13980.1439979729040724No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13450.13853882228610687No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG11410.11752624254903192No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11290.11629020844685105No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT10590.10908000951746258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10490.10804998109897852No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG10400.10712295552234286No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10350.10660794131310083No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10220.10526890436907155No Hit
CAGTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATA10130.10434187879243588No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAATGAATACTACATGAGCTGGATCCGACAGGCTCCAGGGAAGGGG10000.1030028418484066No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9940.10238482479731614No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9870.10166380490437732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9770.10063377648589324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)