FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970847
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA19620.20209157570657377No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17510.18035797607655996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15430.15893338497209136No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15270.15728533950251689No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG14740.15182618888455132No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC14480.14914811499649275No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC13860.14276193880189153No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12720.1310196148311732No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10490.10804998109897852No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG10370.10681394699679765No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT10210.10516590152722313No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9940.10238482479731614No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAATGAATACTACATGAGCTGGATCCGACAGGCTCCAGGGAAGGGG9730.10022176511849962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38400.041.1016541
GGTATCA15150.040.8542941
GAATTCG650.001154882140.68263270
TTCGGGG8600.036.898270
TATCAAC43050.036.479762
GTGGTAT5900.036.1341251
ATCAACG45750.034.300313
TCAACGC46250.033.756644
CAACGCA47300.033.0730175
AACGCAG48050.032.6281856
CGGGATT1101.1186785E-931.20173617
CGCAGAG52200.030.0999018
TGGTATC7050.029.7337462
ACGCAGA53100.029.654347
GCAGAGT53750.029.1042549
CAGAGTA54250.028.7095410
AGAGTAC58950.026.53695911
TTAACGG809.597961E-525.86003135
AGTACTT27200.025.22513
AGTACGG34400.025.03141213
Produced by FastQC (version 0.11.5)