FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462826
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26610.18190816952939037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11100.038.7219281
GTATCAA66650.038.692881
GGTATCA28600.036.789621
TATCAAC74750.034.376252
TGGTATC12400.033.5221442
ATCAACG77750.033.0691033
TCAACGC78000.032.8898354
CAACGCA79600.032.346935
AACGCAG81650.031.5757056
ACGCAGA87100.029.5999587
CGCAGAG88100.029.2250618
AGAGTAC89100.028.93553511
CAGAGTA91250.028.21619410
GCAGAGT92150.027.9034129
GTATAGG2300.025.4150521
AGTACGG59900.024.09691813
GAGTACG59950.024.07612
GAGTACT47850.023.71578212
AGTACTT47950.023.52412813
CGAACTA1803.601599E-1022.91552229
Produced by FastQC (version 0.11.5)