FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110518
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42760.38504553730781493No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAACGCCCCTAAACTCCTGATCTATAAGGCGT34200.3079643913921251No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33400.3007605459794438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31610.28464194186856945No Hit
CCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTACAAAATGGGGTCCCA29770.26807309741940244No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29740.26780295321642694No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28630.25780761770633165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27090.24394021528692014No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGTGCCAGTCAGA26370.237456754415507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25830.2325941587619471No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25710.23151358195004496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.20530959426141673No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22150.19945646986361318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21850.19675502783385773No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGTGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA21450.19315310512751707No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA21100.19000142275946902No Hit
ATATAATAGTTATTTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21100.19000142275946902No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19690.17730464521961822No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19660.1770345010166427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18850.16974060753630288No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18550.16703916550654738No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18140.16334719473254825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17820.16046565656747572No Hit
TCTATAAGGCGTCTAGTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT17600.15848459907898835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17540.15794431067303727No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17440.1570438299964521No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17350.15623339738752548No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17160.15452248410201366No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17010.15317176308713593No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC16940.1525414266135263No Hit
GTTTAGGGGCGTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA16840.15164094593694113No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC16800.15128075366630706No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16590.14938974424547824No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16480.14839921550123455No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAACGCCCCT16220.14605796574211316No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15710.14146551429152882No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15680.14119537008855326No Hit
CTAATACTCTGACTGGCACGGCAAGTGATGGTGACTCTGTCTCCTACAGA15290.13768349544987113No Hit
GTCTCCTTCCGCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGTGCCAGTCAGAGTATTAGTAG15180.13669296670562747No Hit
CAATATAATAGTTATTTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15150.13642282250265192No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14640.1318303710520676No Hit
CCTTATAGATCAGGAGTTTAGGGGCGTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA14330.1290388809546536No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13780.1240862372334352No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13690.12327580462450857No Hit
CCTTCCGCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13190.11877340124158277No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13150.11841320897094869No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13120.11814306476797315No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13110.11805301670031462No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12950.11661224761777837No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12680.11418094979099844No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12520.11274018070846217No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12480.11237998843782811No Hit
ACCTTGATGGGACCCCATTTTGTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCGTTCCCTGGTTTCTG12460.1121998923025111No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12430.11192974809953553No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12430.11192974809953553No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12400.11165960389655999No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAATAGTTATTTGTGGACGTT12270.11048897901699928No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12250.11030888288168224No Hit
GGTATCAGCAGAAACCAGGGAACGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTACAAAATGGGGTCCCAT12190.10976859447573113No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12180.10967854640807265No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12160.10949845027275559No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12020.10823777732553637No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11900.10715720051363417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.10589652756641495No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11710.10544628722812238No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG11710.10544628722812238No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCGCC11620.10463585461919572No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11420.1028348932660254No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11370.10238465292773283No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC11330.10202446065709876No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11280.1015742203188062No Hit
ACTTATTACTGCCAACAATATAATAGTTATTTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11180.10067373964222101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4950.051.2564541
CTTATAC2900.043.7447361
TGGCGTA404.987752E-543.14569538
GTATCAA47350.040.911981
TCGACGT3200.039.6973229
TATACAC3300.039.48313
CTCGACG3300.038.48568728
GGCGTAT459.9179786E-538.39700339
TATCAAC52100.037.31532
ACGAGAC3400.036.2967222
ATCAACG55050.035.440233
CGAGACT3400.035.29483823
TCAACGC56000.034.9614684
AGACTCG3550.034.7802925
CAACGCA56800.034.589795
CGACGTC3700.034.3514130
AACGCAG58300.033.6410146
ATCTCCG3900.033.3877210
CCCACGA3700.033.34623319
GCGTATG751.6400809E-632.27253340
Produced by FastQC (version 0.11.5)