FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874120
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC36720.4200796229350661No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26010.29755639957900515No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCAT24250.27742186427492793No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20610.23577998444149545No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17390.19894293689653597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16600.18990527616345582No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16530.18910447078204365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16050.18361323388093168No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA15640.17892280236123187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15500.17732119159840756No Hit
ATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAA14260.16313549627053495No Hit
GATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA13940.15947467166979362No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13660.15627145014414498No Hit
GCATAGATCAGGGACTTAGGGGCCTTCCCTGGTTGCTGCTGAAACCAGGC13560.1551274424564133No Hit
GTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG13160.15055141170548667No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12920.14780579325493068No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAAGTGACGT12510.14311536173523087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.1430009609664577No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12290.1405985448222212No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCA11580.1324760902393264No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC11550.13213288793300693No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11190.12801446025717292No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10930.12504004026907062No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10880.12446803642520478No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA10870.12435363565643162No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCAGCATCACTT10780.12332402873747313No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT10690.12229442181851463No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10600.12126481489955614No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC10510.12023520798059764No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG10470.11977760490550497No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10430.11932000183041229No Hit
AGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAACCAGGGAAGGCCCCTAAGTCCCTGATCTATG10360.11851919644900015No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10220.11691758568617582No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10040.11485837184825883No Hit
GTGTATTACTGTGCGACCTCGGGGAGTGACTACTACCACGGTATGGACGT9900.11325676108543449No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9840.1125703564727955No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC9480.10845192879696151No Hit
GCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG9410.10765112341554935No Hit
GTCTGCATCTATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGG9330.10673591726536402No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9250.1058207111151787No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9190.10513430650253969No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8890.10170228343934472No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC8830.10101587882670572No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTG8810.10078707728915937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)