FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874120
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC39180.448222212053265No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23590.269871413535899No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCAT23300.26655379124147716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23040.26357937125337483No Hit
GATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA19050.2179334645128815No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC16540.18921887155081682No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15760.18029561158650986No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14790.16919873701551275No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA14490.16576671395231776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14260.16313549627053495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13900.15901706859470097No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCA13570.15524184322518647No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12470.1426577586601382No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11960.1368233194527067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11810.13510730792110923No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11690.13373449869583123No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11360.12995927332631674No Hit
GTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG11100.12698485333821444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11060.12652725026312178No Hit
GCATAGATCAGGGACTTAGGGGCCTTCCCTGGTTGCTGCTGAAACCAGGC11050.12641284949434858No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10930.12504004026907062No Hit
ATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAA10850.12412483411888527No Hit
GTATCAACGCAGAGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTG10610.12137921566832928No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10580.1210360133620098No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC10540.12057841028691713No Hit
GTCCCTGATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGT10490.1200064064430513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10360.11851919644900015No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT9900.11325676108543449No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG9730.11131194801629067No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9710.11108314647874434No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAAGTGACGT9650.11039674186610535No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCAGCATCACTT9610.10993913879101266No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9560.10936713494714685No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC9450.10810872649064203No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA9270.10604951265272503No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8880.10158788267057153No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT8850.10124468036425205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT200.00709292352.23318549
TATAGCG200.00753971251.4324467
TGGTATC4350.048.189942
GTGGTAT4550.046.8457761
TAACGAA4400.046.5833170
GGTATCA13450.044.986331
GTATCAA33300.039.540731
CGAATAA350.001388647239.1888714
TATCAAC38000.034.9075622
CGCATTG400.002674790834.29025714
CTAACGA4550.032.7438269
ATCAACG41350.032.057453
TCAACGC41250.032.024394
CAACGCA41600.031.6525465
ATATCGA450.00432571431.0848255
AACGCAG42800.030.9235576
TTCGGGG5750.029.89694470
CGCAGAG45650.028.9929528
CCGGTGT605.51542E-428.66540329
AGAGTAC46150.028.60453811
Produced by FastQC (version 0.11.5)