FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769108
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.18944023466145196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.10505676706002277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAA250.001784738473.9921770
GTGGTAT5350.040.9238171
GGTATCA16900.040.687341
GTATCAA41300.039.511021
TATCAAC47250.034.6803632
ATCAACG50200.032.6423723
TCAACGC50200.032.6423724
CGATTGT952.6632054E-432.45270570
TGGTATC6800.032.1974142
CAACGCA51300.032.0091255
AACGCAG52800.031.0349856
CTTATAC2550.030.8558561
TATACAC3150.029.3226453
ACGCAGA57600.028.4450387
CGCAGAG58050.028.2245318
AGAGTAC59350.027.66393511
CAGAGTA60650.027.12737510
CGTATGC2050.027.06812541
GCAGAGT62250.026.3202259
TCGTATG1901.8189894E-1225.52248240
Produced by FastQC (version 0.11.5)