FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706883
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56380.33030969316584674No Hit
GGGTTACAGTACCCGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT54440.3189439463630489No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42740.2503979476039072No Hit
CTGTAACCCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC41550.24342617508054154No Hit
GATCTATGCTGCATCCAATTTGCCAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA36790.2155390849870788No Hit
GTCCTGATCTATGCTGCATCCAATTTGCCAAGTGGGGTCCCATCAAGGTT34710.2033531296521203No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34180.20024805449465488No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32250.18894089401558278No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32070.18788634018851907No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATCAGCAGTTATTTAAATTGGTATCAACACAAA31110.18226205311084592No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30770.18027011810416999No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29400.1722437917537406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28930.16949023453862977No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28230.1653891918778264No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC27630.1618740124542807No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26840.1572456928799455No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24750.14500115122126123No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24710.14476680592635818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24590.14406377004164903No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAGGTGACATCCAGATGACCCAGTC23950.14031424532320025No Hit
CCATTCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG23160.13568592574886504No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAGGTGACAT23020.13486571721670437No Hit
GACCTGCACCGTCTCTGGGTTCTCACTCAGCAATACTAAAGTGGGTGTGA22870.13398692236081794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22630.13258085059139965No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTGTGTTGATACCAATTTAAAT22340.13088184720335252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22210.13012022499491763No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTGTGTTGATACCAATT21680.12701514983745224No Hit
ACTTACTACTGTCAACAGGGTTACAGTACCCGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA21280.12467169688842175No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20920.12256258923429432No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA20880.12232824393939128No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACCGGGTA20740.12150803540723061No Hit
ACACAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGCTGCATCCAATTTGCCAAGTGGGGTCCCATCAAGGTT20630.12086358584624723No Hit
CTCCTGCTGCTGACCACCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGAAGGAGTCTGGTCCTGTGCTGGTGAAA20110.11781709701250759No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19820.1161180936244605No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA19670.11523929876857406No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19640.11506353979739677No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19330.11324736376189815No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18980.11119684243149647No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18580.10885338948246599No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18290.1071543860944189No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18140.10627559123853245No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17780.10416648358440503No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17520.10264323916753522No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17500.10252606652008367No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGGGTTACAGTACC17430.10211596225400334No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC17360.101705857987923No Hit
GTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATTCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA17120.10029978621850472No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17080.10006544092360167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)