FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991572
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21300.21481042223862715No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20390.20563307556082663No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT14440.14562734728290028No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12750.12858370345269934No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12000.12101995619077585No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11990.12091910622728354No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11840.11940635677489884No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10920.11012816013360603No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACCGAGGTCAGCTTCAGGGAGA10830.10922051046217522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10730.10821201082725207No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10670.10760691104629821No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10650.10740521111931357No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGCCAACTGGCTGCTCACTTTCGGCGGAGGGA10210.10296781272565178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.039.6768951
GTATCAA33350.039.3491741
TATCAAC37450.034.92812
ATTCGTA500.00284121133.87034669
GTGGTAT7150.033.2391241
TCAACGC39600.033.163724
ATCAACG39900.032.9392973
CAACGCA40350.032.5341645
AACGCAG41200.031.946366
TGGTATC7300.031.1217672
ACGCAGA45450.028.8834657
TTCGGGG5250.028.80459270
CGCAGAG45450.028.7322438
AGAGTAC45550.028.66916311
GCAGAGT47000.027.9309259
CAGAGTA47600.027.57885610
TATTGAT6700.027.47745170
AGTACGG28200.026.56606313
GAGTACG29050.025.90442512
ACGGGGA5950.025.42451516
Produced by FastQC (version 0.11.5)