FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738026
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18150.2459262952795701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.1292637386758732No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9520.12899274551303072No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT8250.11178467967253186No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT7690.10419687111294182No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT7620.10324839504299306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6750.048.6637951
TGGTATC7100.045.7518842
GGTATCA22050.045.467131
GTATCAA56850.040.145221
TATCAAC65950.034.5305252
ATCAACG67750.033.6017073
TCAACGC67700.033.5464554
CAACGCA69000.032.90555
AACGCAG71200.032.128526
CGCAGAG76150.030.1297348
ACGCAGA77900.029.5405357
GCAGAGT78750.029.1783319
AGAGTAC79850.028.69085711
CAGAGTA80500.028.62884510
ACGGGTA2750.028.56332616
TCGTTGC500.00787867327.49472835
GTCTTAC659.0808916E-426.3205621
GAGTACG53000.025.12376212
GTAGTTT5050.024.99771970
CGGGACA1101.2567925E-624.84104217
Produced by FastQC (version 0.11.5)