FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049716
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14060.133940989753419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT150.002124234670.8214361
GTATCAA51000.038.8359831
GGTATCA22050.038.163871
TATCAAC58550.034.06182
TCAACGC61400.032.536474
ATCAACG61550.032.512753
CAACGCA63000.031.8187395
GTGGTAT9650.031.1947351
AACGCAG64750.030.9059436
TGGTATC10150.029.995072
ACGCAGA69500.028.7403417
CGCAGAG71200.028.0527848
AGAGTAC72400.027.72954411
CAGAGTA73400.027.30516210
GCAGAGT73850.027.1387799
GAGTACG46200.024.72564512
AGTACGG46250.024.55101813
CTCGTAT1704.329195E-922.37110539
GAGTACT39550.021.79200612
GTACGGG51850.021.57165714
Produced by FastQC (version 0.11.5)