FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049716
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27980.26654828544101455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56050.038.4756471
GGTATCA25250.038.3051571
GTGGTAT9900.035.212531
TGGTATC9800.033.4650042
TATCAAC65650.032.6791422
TCAACGC69150.030.7951664
ATCAACG69400.030.652593
CAACGCA71100.029.891185
AACGCAG73700.028.9278726
ACGCAGA79300.026.842037
CGCAGAG79400.026.7652638
AGAGTAC81700.026.09527611
CAGAGTA82800.025.748610
GCAGAGT82700.025.7384879
CGCTTAT700.00141717924.38760424
AGTACGG46800.024.12834213
GAGTACG47250.023.96846412
TACGGGC11700.023.62595215
AACCGGT750.002128437622.7411657
GAGTACT47250.021.08069812
Produced by FastQC (version 0.11.5)