FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573394
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.14824012807947068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25650.040.7536931
GGTATCA10350.037.874361
TATCAAC29300.035.9115832
CTCGTAT601.3149687E-534.60025839
ATCAACG30200.034.3859253
TCAACGC30300.034.1589554
CAACGCA31250.033.230565
AACGCAG31900.032.661246
GTGGTAT3800.032.576141
GACGCGT751.7074799E-632.10193626
TGGTATC4100.030.1925222
GCGTAGT802.811399E-630.1139629
ACGCGTA802.8229042E-630.0981927
AGAGTAC35150.029.9217811
ACGCAGA34750.029.8731757
CGCAGAG34800.029.827658
CAGAGTA35950.029.16032210
GCAGAGT36550.028.493559
CGCGTAG854.526215E-628.3277128
TAGCGGC756.317417E-527.4967488
Produced by FastQC (version 0.11.5)