FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511243
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42240.27950501673126027No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40020.2648151223860094No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGATT27630.18282963097264968No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23430.15503793896812093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23030.15239111115816584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22520.14901640570047306No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21040.13922314280363912No Hit
CTCCTGCAGGGCCAGTCAGAGTGCTAGCAGCGACTTAGCCTGGTACCAACAAAAATTTGGCCAGGCTCCCAGGCT20910.1383629237654037No Hit
GTCTATTACTGCCAGCACCGTAACAACTGGGCTCCCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAA20850.13796589959391042No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20690.1369071684699284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18720.12387154150589944No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAGCAG18650.12340834663915731No Hit
GCCTAGAGCCTGAAGATTCTGCAGTCTATTACTGCCAGCACCGTAACAACTGGGCTCCCACTTTCGGCCCTGGGA17200.11381359582807No Hit
GTAATAGACTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA17150.11348274235182562No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAGCAGCGACTTAGCCTGGTA16250.1075273797794266No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16210.10726269699843109No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGAT15910.10527757614096475No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG15910.10527757614096475No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15620.1033586259787473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.042.6211131
GTATCAA65650.041.9047661
TATCAAC72600.037.6354562
ATCAACG78500.034.511253
TCAACGC79400.034.1792644
CAACGCA80850.033.5083665
AACGCAG82050.032.8908966
CGCAGAG89600.030.1958438
ACGCAGA90800.029.759067
AGAGTAC91500.029.71755611
GCAGAGT92900.029.2338169
GTGGTAT11300.029.1781961
CAGAGTA95500.028.6172310
TGGTATC11700.027.8713822
GAGTACG56450.027.2402112
AGTACGG56350.027.16789813
TACGGGT5750.024.43122715
GAGTACT49250.024.2687412
AGTACTT49900.023.74750513
GTATACC1452.1145752E-823.6863231
Produced by FastQC (version 0.11.5)