FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503279
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.18141031117928624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.11047550166011298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA459.909862E-538.3971538
CTACGGA451.0852252E-437.809423
ACGCAGT400.002027681636.2785366
GTATCAA38700.035.259481
GGTATCA17350.033.1458931
TATCAAC43100.032.133622
AACGCAG44750.031.1008876
ATCAACG46150.030.1574133
TCAACGC46450.029.962644
CAACGCA47600.029.2387545
ACGAGAC953.616442E-728.64701722
ACGCAGA48400.028.6034837
CGCAGAG49400.028.0244668
GTGGTAT6050.027.5602551
ACGTAGA1005.575057E-727.26342827
AGCGTCA1005.648235E-727.222783
CGAGACG1005.6580575E-727.21737123
AGAGTAC51200.027.03922811
CAGAGTA51800.026.791710
GCAGAGT51950.026.6488679
Produced by FastQC (version 0.11.5)