FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503279
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11990.2382376375727976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.13213346871218548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAA200.00655315853.28555356
GTATCAA43800.036.9166531
TATCAAC49550.032.32892
AACGCAG49800.032.248636
GGTATCA18800.031.6871381
GTAGCCG901.6947342E-731.23569347
ATCAACG52300.030.6757143
TCAACGC52300.030.6513254
CAACGCA53300.030.1946935
CGCAGAG54000.029.8032828
ACGCAGA54000.029.7404047
TCCGTCA1003.0971241E-629.73851469
CCGTCAT1303.8859827E-529.51997670
GTGGTAT7000.029.1780341
GCAGAGT56450.028.6300759
AGAGTAC56400.028.59525711
TAGCCGT1004.203157E-728.15845348
CAGAGTA57300.028.14611410
TGAGTAC1307.4323907E-926.125564
TAGTGCG700.001096870525.48001959
Produced by FastQC (version 0.11.5)