FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542891
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.13372850166976427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.043.073091
TATGCCG955.456968E-1240.22775343
GTATCAA23350.039.8685461
GTGGTAT2800.039.2590641
TCGTATG901.1641532E-1038.509140
TATCAAC27650.033.6683732
CGGTACC553.1035006E-431.61912745
TCAACGC29750.031.4072514
ATCAACG29650.031.3973223
CAACGCA30300.030.7237825
CGTATGC1251.1641532E-1030.53042441
AACGCAG30850.030.2873826
TGGTATC3700.029.7095622
TCTCGTA1052.2593667E-829.66015838
CGAATTA703.964572E-529.44158615
ACGCAGA33300.027.9507227
CGCAGAG33550.027.7424458
AGAGTAC34200.027.01432811
TAACGGC658.5565087E-426.58848436
TTAACGG658.602938E-426.5638635
Produced by FastQC (version 0.11.5)