FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542891
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14910.27464076582592084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.10333566038118149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26000.041.0521931
GGTATCA9500.040.8229331
GTGGTAT3150.040.3125721
TATCAAC29750.035.6468282
TGGTATC3750.033.862562
TCAACGC32100.032.79314
CAACGCA32700.032.4011085
ATCAACG32550.032.3606033
TTCCCGT851.2630699E-732.301331
AACGCAG33450.031.8796376
ACGCAGA36800.028.79127
CGCAGAG36750.028.6437668
GGACGAG1105.624982E-428.56151470
AGAGTAC37650.027.95905911
CAGAGTA38250.027.69976810
GCAGAGT38600.027.3597769
TCCCGTA1058.0289465E-726.1486972
AGTACGG23350.025.55121213
GAGTACG23600.025.4234912
CGATGAA1250.001186799625.13413270
Produced by FastQC (version 0.11.5)