FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480047
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.14364408697831893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.039.176081
GTATCAA52700.039.115151
TATCAAC60250.034.0422332
GTGGTAT7950.033.7641871
TCAACGC62850.032.9077344
ATCAACG62950.032.8554573
CAACGCA64300.032.2191665
TATGCCG2950.031.74056243
AACGCAG65900.031.4369136
TCGTATG3000.030.00652540
CGTATGC3400.029.5453741
CGAGACG3400.029.3536823
ACGCAGA71550.028.8524287
TGGTATC9450.028.7689532
CGCAGAG73700.028.0107358
CAGAGTA76800.026.96969410
GCAGAGT76950.026.7382729
AGAGTAC78850.026.18124611
ACGAGAC3900.025.59038522
CTTATAC4350.025.3156761
Produced by FastQC (version 0.11.5)