FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516632
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.1192338066554143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2650.048.0140761
GGTATCA9300.042.1535951
GTATCAA22050.038.20941
TGGTATC3400.035.3998832
CGGTATC400.00257331134.55911337
CTAAGCT400.002579457634.54230536
AGTCCGT501.9000536E-434.3751458
TATCAAC25350.033.5067022
TCAACGC27200.032.112754
ATCAACG27550.031.7047863
CAACGCA28450.030.8226955
AACGCAG29550.029.675326
TAGCGGC853.6753117E-629.094664
GCGCTAA500.00775446927.58287633
CGTATAC500.0078541727.5107673
CGTAGCA500.0078541727.5107672
CGCAGAG31750.027.5001168
ACGCAGA32150.027.2675297
AGAGTAC32050.027.1354511
CAGAGTA32900.026.6433510
Produced by FastQC (version 0.11.5)