FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516632
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12820.24814568203285897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT306.427044E-445.826091
GGTATCA9250.041.986611
GTATCAA27150.041.521981
GTGGTAT3100.039.9130481
TATCAAC31350.035.8287432
TGGTATC3600.035.303732
AAACGTA400.002666378434.30965425
ATCAACG33300.033.7143173
TCAACGC34050.032.9461674
CAACGCA34450.032.5478745
AACGCAG35100.032.0428286
AGAGTAC37750.030.24763111
ACGCAGA37300.030.244837
CGCAGAG37550.030.0434658
CAGAGTA38400.029.73562810
GCAGAGT39200.028.8663549
ACGGGTC1254.4055923E-927.43975316
CGAATTA756.406101E-527.43975315
GCGTCTC756.418784E-527.43178612
GAGTACG25650.025.53348412
Produced by FastQC (version 0.11.5)