FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470096
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.14367862625582162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.043.046031
GTATCAA92250.038.9248731
GTGGTAT14600.035.6068341
TATCAAC103250.034.6778832
ATCAACG110050.032.535133
TCAACGC110900.032.2547234
CAACGCA114600.031.3034635
AACGCAG115400.031.0267856
ACGCAGA124600.028.727167
AGAGTAC129200.027.94298411
CGCAGAG128250.027.9358568
CAGAGTA134350.026.87185310
GCAGAGT135800.026.432879
AGTACGG85700.025.17946613
GAGTACG88500.024.38282812
TGGTATC23900.022.6157512
GAGTACT66250.021.87036312
AGTACTT67400.021.54826713
GTACGGG100250.021.42214
TACGGGG48700.020.98878515
Produced by FastQC (version 0.11.5)