FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483535
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30950.2086233219977958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.10690681379273155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9850.044.3168071
GGTATCA30450.043.232711
GTATCAA75600.040.6458971
TGGTATC10500.040.224562
TATCAAC86550.035.4290772
ATCAACG89750.034.029263
TCAACGC90650.033.627454
CAACGCA92700.032.9803165
AACGCAG94400.032.640546
ACGCAGA100500.030.625277
CGCAGAG102600.030.0318438
AGAGTAC105350.029.15030711
CAGAGTA107800.028.58318510
GCAGAGT108000.028.4033099
GAGTACG66650.025.35224512
AGTACGG66700.025.33494813
GAGTACT59150.023.17794412
AGTACTT59450.023.12019313
TACGGGG29900.022.4735515
GTACGGG75150.022.44365914
Produced by FastQC (version 0.11.5)