FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909902
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCA57550.30132436114523153No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGATATGATGTACACTGGTACCAGCACCTTCCAGGAGCAGC53680.2810615413775157No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCACGAACCACTC44030.23053538872675142No Hit
GGATATGATGTACACTGGTACCAGCACCTTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG42610.2231004522745146No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42100.22043015819659856No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40730.21325701528141236No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40660.21289050432954149No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38800.2031517847512595No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38100.19948667523255117No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG37380.1957168482990227No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAGCAGCCCCCAAACTCCTCAT33850.1772342245832509No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32330.16927570105691286No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30260.15843744862301834No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29830.15618602420438327No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28630.14990297931516905No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28530.14937939224106786No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28420.14880344645955657No Hit
GTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC27220.14252040157034235No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT26850.14058312939616796No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGTGGATATTCGGCGGAGGGACCA26180.13707509599969003No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26090.13660386763299895No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25030.1310538446475264No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGATATGATGTACACTGGTACCAG24640.1290118550585318No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24160.12649863710284612No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23990.1256085390768741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.12157691860629498No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCACGAACCACTCA23110.12100097282478367No Hit
TTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCCC23110.12100097282478367No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22720.11895898323578905No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21930.1148226453503897No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG21790.11408962344664805No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20790.1088537527056362No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20580.10775421985002372No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20490.10728299148333265No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT19900.10419382774613567No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19280.10094758788670832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8250.043.782181
GGTATCA26650.043.500551
GTATCAA66600.039.7721021
TGGTATC9250.039.4198342
TATCAAC74600.035.6905142
ATCAACG79900.033.4522173
TCAACGC80350.033.1792454
CAACGCA81500.032.584455
AACGCAG83300.031.9629346
ACGCAGA89200.029.7615327
CGCAGAG91200.029.0334558
AGAGTAC91400.028.93230211
CAGAGTA92950.028.52382910
GCAGAGT95250.027.907279
AGTACGG62150.025.89450313
GAGTACG62300.025.77695812
TACGGGA20000.023.2122915
GAGTACT45450.023.15209812
GTACGGG70550.022.5189314
AGTACTT47000.022.24224313
Produced by FastQC (version 0.11.5)