FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909902
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGATATGATGTACACTGGTACCAGCACCTTCCAGGAGCAGC50490.2643591137136879No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCA47110.24666187060906788No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41820.21896411438911526No Hit
GGATATGATGTACACTGGTACCAGCACCTTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG40550.21231455854803022No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40510.21210512371838972No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36030.18864842279865668No Hit
GTCCTGGGCCCAGTCTGCGCTGACGCAGCCGCCCTCAATGTCCGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG36020.18859606409124655No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34390.18006159478339726No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCACGAACCACTC33990.17796724648699253No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAGCAGCCCCCAAACTCCTCAT30310.15869924216006895No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29760.15581951325251245No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29590.1549294152265404No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28520.14932703353365773No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG28270.1480180658484048No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT26800.14032133585911738No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26560.13906472688127453No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26040.1363420740959484No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25870.13545197606997636No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25820.13519018253292578No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25770.13492838899587517No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGTGGATATTCGGCGGAGGGACCA25740.13477131287364483No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23690.12403777785457056No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23290.1219434295581658No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGATATGATGTACACTGGTACCAG22900.1199014399691712No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGCGCTGACGCAGCCGCCCTCAATGTCCGGGGCCCCAGGGCAGAGGGTCAC22360.1170740697690248No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21570.11293773188362545No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21390.11199527515024332No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG21330.1116811229057826No Hit
GTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC21260.11131461195391178No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20680.10827780692412489No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20230.10592166509066957No Hit
TTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCCC19560.10241363169419164No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19450.10183768591268035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10550.051.1906361
TGGTATC11300.046.5411722
GTATCAA72350.040.3181571
GGTATCA29550.038.9969521
TATCAAC81300.035.895622
ATCAACG88400.033.0350273
TCAACGC89350.032.6188284
CAACGCA90900.032.2396245
AACGCAG92750.031.7437326
ACGCAGA98400.029.9907957
CGCAGAG99550.029.4719928
AGAGTAC99750.029.0344911
CAGAGTA101600.028.77600910
GCAGAGT103800.028.232239
AGTACGG65650.025.82257813
GAGTACG66350.025.65157312
GAGTACT50400.023.62502112
ATAAACG902.1879969E-422.9042843
AGTACTT51850.022.83377513
TACGGGG35600.022.5636715
Produced by FastQC (version 0.11.5)