FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543185
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53200.3447415572339026No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37820.2450775506501165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35450.22971970308161369No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34630.22440601742500088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32500.21060339492672622No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31080.2014016465945431No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29440.19077427528131755No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGAGCATTAGCAGTTACTTAGATTGGTTTCAGCAGAAG28580.18520138544633338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28160.18247974157343416No Hit
GACTTACAGTACCCCTCGGACTTTTGGCCGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT27980.18131332277076306No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCGGCCAAAAGTC27520.17833247471949248No Hit
CTGTAAGTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTTCTGATGGTGAGAGTGAAATCTGTC26650.1726947838399155No Hit
GTACTGTAAGTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTTCTGATGGTGAGAGTGAAAT26640.17262998279532266No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGAAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGA26610.17243557966154416No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26390.17100995668050167No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT26040.16874192011975234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25480.16511306162255335No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23460.1520232506147999No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGA23150.1500144182324219No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22840.1480055858500439No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22560.1461911566014444No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA21740.14087747094483163No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21200.1373782145368183No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCGGCCAAAAGTCCGAGGGGTA20530.13303654454909813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20070.13005569649782756No Hit
CTTACAGTACCCCTCGGACTTTTGGCCGGGGGACCAAGGTGGAGATCAAA20060.1299908954532347No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19060.1235107909939508No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18890.12240917323587255No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT18750.12150195861157281No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.11955792727378765No Hit
GTGTCTGGTGGCTCCATCAACAGTGCTGAATACTACTGGAGTTGGATCCG18290.11852111056030222No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCA18120.11741949280222397No Hit
GTAATACATGGCCGTGTCTGCGGCAGTCACAGAGCGCAGGTTCAGGGAGAACTGGCTGTTGGAGGTGTCTACTGA18110.11735469175763114No Hit
GATTTCACTCTCACCATCAGAAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGACTTACAGTACC18000.11664188026710991No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17550.11372583326043216No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17480.11327222594828229No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCAACAGTGCTGAATACTACTGGA17110.11087458729834726No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16710.1082825455146337No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16460.10666251939981274No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16420.10640331522144136No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGCTGAAACCAATCTAAGT16220.10510729432958459No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGCTGAAACCAATC16200.10497769224039892No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15990.1036168703039493No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15920.10316326299179943No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15780.10225604836749969No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15610.10115443060942143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6250.049.496521
TGGTATC6750.044.8116652
GTATCAA50600.041.8448871
GGTATCA19700.041.177311
CTACGCT2250.038.23141528
TATCAAC57750.036.723612
ATCAACG61000.034.654333
ACGAGAC2600.034.37815522
TCAACGC61650.034.23324
CAACGCA62950.033.5262375
AACGCAG63700.033.1315046
ACTACGC2750.031.27720527
CGCAGAG68800.030.5147728
AGAGTAC69700.030.26864611
ACGCAGA71250.029.6101687
GCAGAGT72000.029.254019
CAGAGTA73650.028.69192910
TACGCTG3000.028.68472929
CGAGACT3250.027.50341623
TATACAC3800.027.1362533
Produced by FastQC (version 0.11.5)