FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543185
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49410.3201819613332167No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36530.23671821589764025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31090.20146644763913593No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29860.19349591915421677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28820.18675661051656153No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28640.1855901917138904No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGAGCATTAGCAGTTACTTAGATTGGTTTCAGCAGAAG28190.18267414470721266No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27530.17839727576408532No Hit
GACTTACAGTACCCCTCGGACTTTTGGCCGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26730.1732131921966582No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCGGCCAAAAGTC25900.1678347054954526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25140.16290982610639684No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT24860.16109539685779736No Hit
GTACTGTAAGTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTTCTGATGGTGAGAGTGAAAT24850.1610305958132045No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGAAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGA24260.15720733418222702No Hit
CTGTAAGTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTTCTGATGGTGAGAGTGAAATCTGTC24220.15694813000385566No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22930.14858879525137947No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22460.14554314615551603No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22400.145154339887959No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGA21980.14243269601505976No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21920.14204388974750273No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA21540.13958145005297484No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20130.13044450276538458No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20110.13031490067619889No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT19960.12934288500730634No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19450.12603803173307151No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCGGCCAAAAGTCCGAGGGGTA18860.12221477010209406No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18720.1213075554777943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18520.12001153458593752No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCA18310.11865071264948791No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17930.11618827295496004No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17660.11443864475095339No Hit
CTTACAGTACCCCTCGGACTTTTGGCCGGGGGACCAAGGTGGAGATCAAA17490.11333702699287514No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17260.11184660296723982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16940.10977296954026898No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT16930.10970816849567615No Hit
GATTTCACTCTCACCATCAGAAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGACTTACAGTACC16860.10925456118352626No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16840.10912495909434061No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16730.10841214760381937No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16670.10802334133626233No Hit
GTGTCTGGTGGCTCCATCAACAGTGCTGAATACTACTGGAGTTGGATCCG16490.10685692253359123No Hit
GTAATACATGGCCGTGTCTGCGGCAGTCACAGAGCGCAGGTTCAGGGAGAACTGGCTGTTGGAGGTGTCTACTGA15940.10329286508098512No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15790.10232084941209252No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15510.10050642016349302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)