FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819124
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.15443327261806514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.042.7459371
GTATCAA36500.039.6806681
TCGCGTA350.00133356539.51255838
GTGGTAT4550.037.0488241
ATCGCGT400.002577463234.55016737
TATCAAC42500.034.3215332
TGGTATC4950.034.0549772
CGTATGC1056.0936145E-1033.01628541
TCAACGC44250.032.9661944
ATCAACG44350.032.8918653
CAACGCA44600.032.7846345
AACGCAG46700.031.5313916
CGCGTAT450.004552116630.76219739
ACGAGAC1950.029.99007222
ACGCAGA49950.029.4690027
CGCAGAG50150.029.4200558
TATGCCG1352.8194336E-1028.30299643
AGAGTAC52400.028.02381511
CAGAGTA52950.027.73272710
GCGTATG500.00758065327.71321540
Produced by FastQC (version 0.11.5)