FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819124
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.23647213364521125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.11048388278209403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6250.044.5099031
TGGTATC6450.043.6355172
GTATCAA42600.040.632411
GGTATCA17450.038.9691961
GCCGTTA901.3096724E-1038.0878215
TATCAAC49100.035.0221022
ATCAACG50850.033.5283743
TCAACGC50950.033.37284
CAACGCA52100.032.8155255
AACGCAG54150.031.6997456
CGCAGAG57750.030.02038
ACGCAGA57550.029.8864887
AGAGTAC59500.028.964611
CAGAGTA61250.028.30485310
GCAGAGT61500.028.1897959
CCGTTAA1103.889363E-828.05162816
GAGTACG31950.027.02378712
AGTACGG31750.026.98642513
TACGGGG13550.025.04520215
CGAACTA1251.3131648E-724.74134829
Produced by FastQC (version 0.11.5)