FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925217
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17000.18374067921363313No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13320.1439662263015055No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA12370.13369836481603775No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG11640.12580832388509938No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG11060.11953952424134014No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG10920.11802636570663963No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10490.11337880735005951No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10330.11164948331040178No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC10190.11013632477570127No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9950.10754233871621469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.10635342843894999No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9820.1061372629339928No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC9710.1049483526567281No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT9390.10148970457741266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.044.539231
TCGTATG651.1050361E-842.60261540
GTATCAA30450.037.8408241
CGTGTAA400.002522401534.70310647
TATCAAC34800.033.3089872
GTGGTAT5250.032.198691
ATCAACG36750.031.5415733
TCAACGC36950.031.4659144
CAACGCA37950.030.727685
TGGTATC5650.030.5297282
ACGAGAC1251.2551027E-1030.3538422
AACGCAG38800.030.0545256
CGAGACG1301.9826984E-1029.18480923
CGCAGAG42250.027.7516788
ACGCAGA42700.027.4592157
GCAGAGT44150.026.4792739
CGTATGC1057.4298805E-726.3859341
CAGAGTA44800.026.17206410
GCGTTGT5150.025.11398762
TCGGATA4750.024.70441429
Produced by FastQC (version 0.11.5)