FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600342
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT65210.2507747057887001No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGTTGTTTATTTGCAGAGACA62430.24008380436111865No Hit
GTATATTACTGTGCGAGAGAAGCGGGCTACTATGGTTCGGGGAATTATTA52230.20085819480668313No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT50700.1949743533735178No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46770.1798609567510735No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCTCAAGTATTAGTGATACTGGTGGT46260.17789967627335174No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT41020.15774848077675935No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40430.15547954845939493No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39850.1532490726219859No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38980.14990335886587225No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38180.14682684046944594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37230.1431734748736897No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT36780.14144293327569987No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG35030.13471304928351732No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT35020.134674592803562No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC33300.1280600782512454No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG33170.12756014401182614No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC32440.12475282097508714No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG31750.12209932385816942No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30580.11759991570339595No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA28950.11133150947067731No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA28950.11133150947067731No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT28800.11075466227134739No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC28300.10883183827358094No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT28170.10833190403416167No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT28090.10802425219451903No Hit
TCCCTGGAGAGTGGCGGACCCAGCTCATGGCATAGCTGCTAAAGGTGAAT27670.10640908003639521No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCACTCTCCAGGGAAGGGG27620.10621679763661858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27150.10440934307871812No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC26350.1013328246822918No Hit
GTATATTACTGTCAGCACTATGGTATCTCACCTCGGACCTTCGGCCAAGGGACACGCCTGGAGATTAAACGAA26350.1013328246822918No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA26240.10090980340278317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)