FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755353
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16980.22479555916240487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6200.043.7905771
ACCGATT600.001022526241.7000870
GTATCAA35700.038.5067671
GGTATCA15800.036.977461
TGGTATC7350.036.9144942
TATCAAC42000.032.4637262
TCAACGC42900.031.504894
ATCAACG43350.031.3610423
CAACGCA44450.030.388185
AACGCAG44750.030.2610746
ACGCAGA48350.028.0788277
CGCCATA500.00713867428.05606755
CGCAGAG48650.027.9761478
AGAGTAC49950.027.24804111
CGCGGTA906.5116255E-627.03508443
CAGAGTA50700.026.84496310
GCAGAGT51500.026.561099
GAGTACG31550.024.23180412
AGTACGG31600.023.9764813
GAGTACT27100.022.13854212
Produced by FastQC (version 0.11.5)