FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304424
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.10840144009670721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG800.056.34702340
GTCGTAT252.5069658E-455.4525739
TTTCGAC252.6133744E-454.9880410
CGTTAGG200.0068692852.649658
AGCGCTA200.006942552.5086654
ATTCCGC200.00725068851.93517738
CTCGTAT707.2759576E-1249.51122739
TATACAC1250.044.0193563
CGTATGC1100.041.0068441
GTGGTAT1350.040.7586631
GGTATCA5500.040.642871
TCTCCGA1200.040.09544811
CCCACGA1150.038.8630119
GTATCAA11700.038.799111
TCTCGTA852.8521754E-936.66012238
ATGCCGT1300.034.78462244
TATGCCG1300.034.75573343
CCCGAAA400.002634231534.390121
ACGAGAC1300.034.3844722
TTCGACC400.002642737534.36752711
Produced by FastQC (version 0.11.5)