FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909192
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31050.16263424527234557No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA25390.13298819605361847No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22280.1166985824369681No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19690.10313263411956472No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCCCTCACTTTCGGCCCTGGGA19230.10072323789330774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.041.2247121
GTATCAA79450.040.1031
TGGTATC11650.038.3952262
GTGGTAT11900.037.5886041
TATCAAC88750.035.9006582
ATCAACG94250.033.8056563
TCAACGC94900.033.465344
CAACGCA96250.033.0317085
AACGCAG98250.032.3943256
CGCAGAG108200.029.500728
AGAGTAC110300.028.90711411
CAGAGTA112250.028.46622510
GCAGAGT113150.028.1790059
ACGCAGA117650.027.0726627
AGTACGG69000.025.67193813
GAGTACG70350.025.27708412
CGATTGT2501.1317752E-825.11968670
TATACGG12200.024.8189075
TCGTATG1301.7976345E-723.95398940
ATACGGC12650.023.120026
Produced by FastQC (version 0.11.5)