FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073527
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28890.2691129333496037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58800.040.8554041
GTGGTAT8400.039.9811741
GGTATCA23800.037.8693281
TATCAAC66150.036.2404252
ATCAACG70150.034.153263
TCAACGC70400.033.9675254
CAACGCA71350.033.5491375
AACGCAG71800.033.4817546
TGGTATC10350.031.7655162
ACGCAGA76900.031.2612467
CGCAGAG78800.030.8546568
AGAGTAC80650.030.23169111
GCAGAGT81300.029.9479269
CAGAGTA81500.029.91639510
TACGATT500.00800998627.40287427
GAGTACG50850.026.3616412
AGTACGG50850.026.02539413
TACGGGG20700.024.61691715
GAGTACT44450.024.54122412
AGTACTT44800.024.27316513
Produced by FastQC (version 0.11.5)