FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827482
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.11855242772652455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.039.776821
GTATCAA31900.039.6819271
TATCAAC34400.036.898062
TCAACGC36350.034.7293974
ATCAACG36700.034.491923
CAACGCA37450.033.617465
AACGCAG38850.032.494566
AATCGCG450.004590205430.70988736
GTGGTAT4950.030.5761681
CGCAGAG42100.029.7992348
ACGCAGA42300.029.7395947
AGAGTAC43400.028.74823811
CAGAGTA44600.028.05180710
ATATGCG500.007235883327.97877754
GCAGAGT45600.027.5120129
TGGTATC5400.026.1171462
AGTACGG26050.025.06918313
GAGTACG26250.025.00911712
TACGGGC6850.024.08781815
CGGGCTA1151.7500606E-623.91471517
Produced by FastQC (version 0.11.5)