FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827482
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13780.16652930214796213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.041.418671
GTATCAA37900.039.2681771
GTGGTAT4850.035.4340131
TATCAAC42700.034.5131652
TCAACGC44700.032.991874
ATCAACG45050.032.8414573
CAACGCA46350.031.809715
AACGCAG47300.031.38646
CGCGAAA550.004703878430.555969
TGGTATC5550.030.3290252
CGCAGAG50050.029.5933698
ACGCAGA50150.029.5343577
AGAGTAC51100.028.64980911
CAGAGTA52300.028.12356410
GCAGAGT53500.027.5568399
GAGTACG31200.025.0535212
AGTACGG30800.024.9366613
CGCTGAT700.001383287324.48840112
TGCGATA700.001383287324.48840112
GAGTACT28700.023.77166712
Produced by FastQC (version 0.11.5)