FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643206
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17700.2751840001492523No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14700.22854264419175196No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14300.22232379673075192No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12940.2011797153633517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10690.1661986983952264No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGAGA8540.13277239329235113No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8080.12562071871220107No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8030.12484336277957607No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA7710.11986828481077602No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7460.11598150514765099No Hit
GTGTATTACTGTGCGAGAGTACCGGCTGCGATGACATTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTC6700.10416569497175088No Hit
GTCTCTGGTGGCCCCATCAGTAGTCACTACTTGAGCTGGATCCGGCAGCC6660.10354381022565089No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6640.10323286785260087No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6510.10121174242777586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTA200.007477184851.5393413
GTGGTAT3100.049.946671
TGGTATC3550.044.5672872
GTATCAA26750.042.3182641
GGTATCA10450.041.8160631
TATCAAC30400.036.996522
ATCAACG32400.034.5985533
TCAACGC32650.034.5254634
CAACGCA33200.034.049085
AACGCAG34200.033.1513866
CGCAGAG36100.031.4065728
GGCGCGT450.00464658230.63232232
AAACGAT450.00470357730.55608222
CAGAGTA37050.030.50854510
GCAGAGT38100.029.7579359
ACGCAGA38300.029.6922457
CGGGACT953.3096512E-728.9411144
CGGGTCG605.537872E-428.644117
CGGGATA1202.7885108E-928.644117
AGAGTAC40100.028.1880711
Produced by FastQC (version 0.11.5)