FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821852
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25040.30467772786341096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.26987827492054534No Hit
GATCTATGCTGCATCTATTTTGGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA20450.24882825618237833No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18140.22072100572852532No Hit
GTAGGAGACAGAGTAACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAA16850.20502474898156844No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16630.20234786798596344No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16540.20125278030594315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16170.19675075317697102No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15890.19334381372801915No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14600.17764755698106227No Hit
GGTTAATGGTTATCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14470.1760657636654775No Hit
GTCTACAGGTTAATGGTTATCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC13790.16779176786088007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12810.15586747978954849No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12680.15428568647396368No Hit
GAGTAACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAACCCGGGAAAG12480.1518521582961409No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12390.15075707061612068No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12210.14856689525608016No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11560.14065792867815616No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11220.13652093077585745No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTAACCATCACTT11000.1338440497802524No Hit
CCATTAACCTGTAGACAGTAATAAGTTCCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC10710.13031543392240938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.12934202265128028No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10350.1259350832023284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9900.12045964480222716No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9530.11595761767325503No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9530.11595761767325503No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9400.11437582435767024No Hit
CCTGTAGACAGTAATAAGTTCCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATC9390.1142541479487791No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAACTGGTTCTTGGACGGGTCAACTGA9090.11060385568204495No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCGGGTTTCCGCTGATACCAGCC9030.10987379722869813No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTCTGGAACTTATTACTGTCTACAGGTTAATGGTTAT8960.10902206236646013No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8570.10427668241970575No Hit
GTCTGCATCTGTAGGAGACAGAGTAACCATCACTTGCCGGGCAAGTCAGG8420.10245153628633866No Hit
TCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG8310.10111309578853614No Hit
AGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAACCCGGGAAAGCCCCTAAGCGCCTGATCTATG8270.10062639015297158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)