FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747006
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25750.3447094133112719No Hit
GATCTATGCTGCATCTATTTTGGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA21050.28179157864863197No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20010.26786933438285637No Hit
GTAGGAGACAGAGTAACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAA16470.22048015678588928No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16170.2164641247861463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15990.2140545055863005No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15650.20950300265325847No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15540.20803045758668606No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14940.1999983935872001No Hit
GTCTACAGGTTAATGGTTATCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC14310.19156472638773986No Hit
GGTTAATGGTTATCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14210.1902260490544922No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13590.1819262495883567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.17911502718853664No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13100.1753667306554432No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12800.1713506986557002No Hit
GAGTAACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAACCCGGGAAAG12530.16773626985593154No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTAACCATCACTT11390.15247534825690823No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCGGGTTTCCGCTGATACCAGCC10950.14658516799061855No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10830.14497875519072137No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10460.14002564905770504No Hit
CCTGTAGACAGTAATAAGTTCCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATC10300.13788376532450877No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10120.135474146124663No Hit
CCATTAACCTGTAGACAGTAATAAGTTCCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC10080.1349386751913639No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9580.12824528852512562No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9380.1255679338586303No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAACTGGTTCTTGGACGGGTCAACTGA9020.12074869545893877No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT8990.12034709225896445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.11954388585901586No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8710.11659879572587101No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8420.11271663145945279No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8390.1123150282594785No Hit
GTCTGCATCTGTAGGAGACAGAGTAACCATCACTTGCCGGGCAAGTCAGG8210.10990540905963272No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8110.10856673172638506No Hit
TCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG8020.10736192212646217No Hit
AGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAACCCGGGAAAGCCCCTAAGCGCCTGATCTATG8010.1072280543931374No Hit
GTCTATTACTGTGCGAGAGATATTGTAGTCATGGTAGCTTCTTCTTTCAACTACCAATACTACGGAATGGACGT7960.10655871572651358No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTCTGGAACTTATTACTGTCTACAGGTTAATGGTTAT7840.10495230292661638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23500.041.1724051
GTGGTAT2800.038.1216281
CTCGTAT852.8903742E-936.62710639
TATCAAC27000.035.835242
TTCGACA400.00252750134.6879444
ATCAACG28650.033.7714273
TATGCCG1253.6379788E-1233.28470243
CGTATGC1253.6379788E-1233.25107641
TCAACGC29350.033.0832984
CAACGCA29850.032.6444855
ACGAGAC1500.032.13048622
AACGCAG30800.031.749396
TCGTATG1251.200533E-1030.46373440
TCGCTAA1151.711669E-929.9834131
CGCAGAG32400.029.9569388
AGAGTAC32900.029.60628111
ACGCAGA34150.028.5225967
CTTATAC1700.028.3561251
GCAGAGT34250.028.3388259
CTCGCTA1353.092282E-1028.08238630
Produced by FastQC (version 0.11.5)