FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747006
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24730.3310549045121458No Hit
GATCTATGCTGCATCTATTTTGGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA18340.2455134229176205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16060.2149915797195739No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15800.21151101865312993No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15190.20334508692031925No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14340.19196632958771417No Hit
GTAGGAGACAGAGTAACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAA14250.19076151998779128No Hit
GTCTACAGGTTAATGGTTATCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC13410.17951663038851093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13280.17777634985528898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12700.17001202132245255No Hit
GGTTAATGGTTATCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12670.16961041812247826No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12580.16840560852255537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12230.16372023785618858No Hit
CCTGTAGACAGTAATAAGTTCCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATC11920.15957033812312083No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11880.15903486718982177No Hit
CCATTAACCTGTAGACAGTAATAAGTTCCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC11320.15153827412363488No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11150.14926252265711387No Hit
GAGTAACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCGGAAACCCGGGAAAG11030.1476561098572167No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10890.14578196159066995No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10240.1370805589245602No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10180.13627735252461157No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9440.12637114025857893No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9390.12570180159195507No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9220.12342605012543406No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTAACCATCACTT9200.12315831465878455No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCGGGTTTCCGCTGATACCAGCC9130.12222124052551117No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9090.12168576959221211No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8850.11847294399241774No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGAACTGGTTCTTGGACGGGTCAACTGA8800.1178036053257939No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8570.1147246474593243No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8370.11204729279282898No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8040.1076296575931117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8010.1072280543931374No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTCTGGAACTTATTACTGTCTACAGGTTAATGGTTAT7880.10548777385991545No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7810.10455069972664208No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7680.10281041919342013No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7610.10187334506014678No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT7550.10107013866019816No Hit
GTGTAGAGATAACCATTAACCTGTAGACAGTAATAAGTTCCAGAATCTTC7540.10093627092687342No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7540.10093627092687342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)