FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717492
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24700.34425470946017517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29600.042.160621
TACGGAT350.001256672539.9919950
GGTATCA11050.038.267921
TATCAAC35050.035.5701522
GTGGTAT4450.034.7651861
ATCAACG36650.033.9022753
TCAACGC36800.033.7311254
CAACGCA37150.033.3024525
AACGCAG38550.032.0907866
TGGTATC4850.030.4504622
ACGCAGA40950.030.042647
CGCAGAG41350.029.5862738
AGAGTAC42550.028.59080311
CAGAGTA43900.027.94576810
GCAGAGT44050.027.8506079
GTGCGAA500.00799104727.41495511
AGTACGG25000.024.40271213
GAGTACG25150.024.25378812
ACGGGTA1701.8007995E-1024.20316116
GAGTACT25450.023.96788812
Produced by FastQC (version 0.11.5)