FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920427
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.38960178265087836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18380.19968992652323325No Hit
ATATTATACCATTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16760.18208939981117458No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT14570.15829609518191015No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGGTGCTC14400.15644912632941016No Hit
GTATTACTGTCATCAATATTATACCATTCCTCGGACTTTTGGCCAGGGGA13770.14960447705249846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12440.13515466191235156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.043.2133521
GTATCAA60900.041.9389841
TGGTATC5900.041.7808072
GTGGTAT6200.040.3113671
TTCGCGA1103.7034988E-739.02526570
TATCAAC69500.037.0942462
ATCAACG75450.034.4866263
TCAACGC76000.034.2370534
CAACGCA77850.033.5553935
AACGCAG79150.033.090776
ACGCAGA84900.030.7169767
CGCAGAG88400.029.5008058
AGAGTAC89350.029.11053811
CAGAGTA91100.028.66403810
ACGGGTC2150.028.65423416
CGAGATA605.6767825E-428.52467520
GCAGAGT91500.028.5013249
AATCTCG2600.026.5090736
AGTACGG54100.026.12666513
GAGTACG54600.026.01277412
Produced by FastQC (version 0.11.5)