FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920427
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39190.4257806431145544No Hit
ATATTATACCATTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17640.19165017975352744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.16166409720705716No Hit
GTATTACTGTCATCAATATTATACCATTCCTCGGACTTTTGGCCAGGGGA13180.14319440868205735No Hit
GTATAATATTGATGACAGTAATACACTGCCACATCTTCAGGCTGCAGGCGGCTGATGGTGAGAGTGAAAT11150.12113942767867523No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11100.12059620154558699No Hit
CAGTAATACACTGCCACATCTTCAGGCTGCAGGCGGCTGATGGTGAGAGT10710.1163590377074988No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGGTGCTC9580.10408212709970481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.044.3272671
GTATCAA64400.043.3088951
TGGTATC6850.041.4039842
GGTATCA23350.040.8404161
TATCAAC70700.039.390622
ATCAACG76100.036.698233
TCAACGC76000.036.6816444
CAACGCA78150.035.784025
AACGCAG79350.035.3698776
AGTGCGT400.002349924835.20774554
ACGCAGA85600.032.7873847
CGCAGAG88550.031.6950878
AGAGTAC89650.031.2267211
CAGAGTA91700.030.7550810
GCAGAGT91700.030.6435139
CGATCAG904.7960784E-628.12045366
AGTACGG55200.026.8739113
GAGTACT49250.026.72480612
GAGTACG55700.026.69099612
AGTACTT49150.026.22701813
Produced by FastQC (version 0.11.5)